FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005110319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292218
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACAGCTCCAGCAATCAGAACTACTTAGCTTGGTACCAACAGAA251900.5868760626790158No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGA183540.42761108592340835No Hit
ATATTACTGTCAGCAATATTTTAGTACTTCCCCCACTTTCGGCCCTGGGA181390.42260202068021707No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT164750.3838341854957041No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC155730.362819409452176No Hit
CTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCT143520.3343725784664246No Hit
GTATATTACTGTCAGCAATATTTTAGTACTTCCCCCACTTTCGGCCCTGG140150.3265211599224457No Hit
GTATATTACTGTGCGAGAGGGGGTCCCAGCTCGCAGTGGTTCGACCCCTG124000.2888949256538228No Hit
GTAATATACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGA121810.2837926684991303No Hit
CAGTAATATACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGT104400.24323088901821854No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC90140.21000797256802892No Hit
CTATAAGTCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCGA89430.20835381613888204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT80710.1880379794316132No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG75110.1749911118214406No Hit
GTACTAAAATATTGCTGACAGTAATATACTGCCACATCTTCAGCCTGCAG74830.17433876844093193No Hit
GTATAGAACACTCTGGCTGGACTTGCAGTTGATGGTGACCCTCTCGCCCA73970.1723351423436554No Hit
ATTTTAGTACTTCCCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA72270.16837448610485303No Hit
ATATACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGAACT71280.1660679862951975No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC71100.16564862269344194No Hit
TTTTAGTACTTCCCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC69970.1630159511935321No Hit
ATTCAGTGGTAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAACC61940.14430767495965954No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT61110.14237394279600898No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC60220.14030042276510654No Hit
GTTTGGGAGAGTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTGA59650.13897243802621395No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA57930.13496518583166092No Hit
ATATTACTGTGCGAGAGGGGGTCCCAGCTCGCAGTGGTTCGACCCCTGGG55710.12979303474334247No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT55230.12867473180532768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG54640.1273001511106845No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA53070.1236423685842611No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG52430.12215129800024138No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG51800.12068352539409695No Hit
GCCGTATATTACTGTGCGAGAGGGGGTCCCAGCTCGCAGTGGTTCGACCC51790.12066022741622164No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC51080.11900607098707475No Hit
ATACAGCTCCAGCAATCAGAACTACTTAGCTTGGTACCAACAGAAACCAG50620.11793436400481057No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA49860.11616371768628714No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAGCATCTGTGGTTCTTCCTTCT49140.11448626327926494No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAGCTGGA48560.11313498056249706No Hit
ACTTAGCTTGGTACCAACAGAAACCAGGACACTCTCCCAAACTGCTCATT47120.10978007174845265No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG45760.10661154675741073No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC44750.10425845099200459No Hit
GTCCCTGACCGATTCAGTGGTAGCGGGTCTGGGACAGATTTCACTCTCAC44270.1031401480539898No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT43400.10111322397883798No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)