FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292218
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACAGCTCCAGCAATCAGAACTACTTAGCTTGGTACCAACAGAA231650.5396976574815165No Hit
ATATTACTGTCAGCAATATTTTAGTACTTCCCCCACTTTCGGCCCTGGGA190230.4431974331219896No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGA181210.42218265707846153No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT166490.3878880336460077No Hit
GTATATTACTGTCAGCAATATTTTAGTACTTCCCCCACTTTCGGCCCTGG140830.32810542241796664No Hit
CTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGCATCCGGGGTCCCT138690.3231196551526507No Hit
GTAATATACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGA138070.32167518052438154No Hit
GTATATTACTGTGCGAGAGGGGGTCCCAGCTCGCAGTGGTTCGACCCCTG131610.3066246868169324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC124630.2903626982599672No Hit
CAGTAATATACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGT119310.2779681740303032No Hit
CTATAAGTCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCGA115020.26797334152179597No Hit
GTACTAAAATATTGCTGACAGTAATATACTGCCACATCTTCAGCCTGCAG80590.1877584036971095No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT78000.18172422742740466No Hit
GTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC77580.18074571235664172No Hit
TTTTAGTACTTCCCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC75950.17694814196296646No Hit
ATATACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGAACT74850.17438536439668254No Hit
ATTTTAGTACTTCCCCCACTTTCGGCCCTGGGACCAAAGTGGATATCAAA73960.17231184436578012No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG73760.17184588480827395No Hit
ATTCAGTGGTAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAACC59980.13974127129609912No Hit
ATATTACTGTGCGAGAGGGGGTCCCAGCTCGCAGTGGTTCGACCCCTGGG59510.13864626633595964No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59260.13806381688907693No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA57390.13370709502639427No Hit
GTATAGAACACTCTGGCTGGACTTGCAGTTGATGGTGACCCTCTCGCCCA57210.13328773142463873No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG54430.126810893575303No Hit
GTTTGGGAGAGTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTGA53270.12410832814176725No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT53150.12382875240726356No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA53030.12354917667275987No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52170.12154555057548334No Hit
GCCGTATATTACTGTGCGAGAGGGGGTCCCAGCTCGCAGTGGTTCGACCC51070.11898277300919943No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG50780.1183071316508155No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT50750.11823723771718958No Hit
ATACAGCTCCAGCAATCAGAACTACTTAGCTTGGTACCAACAGAAACCAG48230.11236614729261188No Hit
GACTTATAGTTGGTGCTCCCACTGTGATGGATCCACGCAATCCACTCCAG48210.11231955133686126No Hit
ACTTAGCTTGGTACCAACAGAAACCAGGACACTCTCCCAAACTGCTCATT47680.11108475850946993No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46340.10796282947417861No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46030.10724059216004406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45930.10700761238129097No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC45300.10553983977514655No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGTAGTTACTACTGGAGCTGGA45030.10491079437251324No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG43960.10241791073985523No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGG43710.10183546129297254No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG43660.10171897140359598No Hit
GTCCCTGACCGATTCAGTGGTAGCGGGTCTGGGACAGATTTCACTCTCAC43160.10055407250983059No Hit
CCATATCAGTAGACACGTCGAAGAACCAGTTCTCCCTGAAGGTGACCTCT43140.10050747655407997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG43110.10043758262045403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)