FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165669
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTATTCATTTGCAGATACA169030.32721802345446444No Hit
GTGTATTACTGTGCGAGAGATGGGTATAGTGGCTACGAACGCCATTTTGA120290.2328643201877627No Hit
CTATAATATGACTTGGGTCCGCCAGGCTCCAGGGCAGGGACTGGAGTGGG117190.22686316138335613No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC78090.15117112614067993No Hit
GTAGTAGTGATGTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTA67930.13150281212365716No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT61680.11940370163090201No Hit
ATCCTATGCAGACTCAGTGAAGGGCCGATTCGCCGTCTCCAGAGACAACG60170.11648055653585238No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA58220.11270563406211276No Hit
TTACACATCCTATGCAGACTCAGTGAAGGGCCGATTCGCCGTCTCCAGAG55140.10674319241128302No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG53890.104323370312732No Hit
CATTAGTAGTGGTACTCTTTACACATCCTATGCAGACTCAGTGAAGGGCC53080.10275532559287093No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA52510.10165188671593167No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT52310.1012647151801635No Hit
TTATGATAGTGGCCGGCCCTCAGGGAGCCCCGAGCGCTTCTCTGGCTCTA52220.10109048798906783No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAACATCACTA52080.10081946791403011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)