FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165669
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGACTCTCAGGCTATTCATTTGCAGATACA158890.3075884265910185No Hit
GTGTATTACTGTGCGAGAGATGGGTATAGTGGCTACGAACGCCATTTTGA122010.23619399539536895No Hit
CTATAATATGACTTGGGTCCGCCAGGCTCCAGGGCAGGGACTGGAGTGGG119960.23222548715374522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80510.15585590172347474No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC79390.15368774112317302No Hit
GTAGTAGTGATGTTGTGGTATTCGGCGGAGGGACCAAGGTGACCGTCCTA62840.12164929653835736No Hit
ATCCTATGCAGACTCAGTGAAGGGCCGATTCGCCGTCTCCAGAGACAACG61470.11899717151834545No Hit
TTACACATCCTATGCAGACTCAGTGAAGGGCCGATTCGCCGTCTCCAGAG57380.11107951361188648No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT57060.11046003915465741No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG55890.10819508567041364No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT54600.10569782926470898No Hit
CCTATGTGCTGACTCAGCCTCCCTCAGTGTCAGTGGCCCCAGGAAAGACG54140.1048073347324422No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACAACATCACTA52620.10186483106060416No Hit
CATTAGTAGTGGTACTCTTTACACATCCTATGCAGACTCAGTGAAGGGCC51930.100529089262204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)