FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4203492
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAA271220.6452254458911781No Hit
GTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGA165170.3929352072039152No Hit
CTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGA144090.3427864261428355No Hit
ATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA130160.3096473122822644No Hit
GTGTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTACTG129080.3070780198939358No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTAGTTACTACTGGGGCTGGATCCG118990.28307416785853284No Hit
ACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTC101630.24177517169058485No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC101360.24113284859350273No Hit
ACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTCCGTA80970.19262555989163294No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA78730.18729665716028485No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT76360.18165848775256382No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC74880.17813760559078023No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATGCCAGTG73750.17544936448076978No Hit
ATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGT73350.17449777470731476No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA69170.16455366157470977No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT67180.15981950245177104No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT62240.14806736874960152No Hit
CTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG61950.14737746616384662No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA61880.147210937953492No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC58950.14024054286293394No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG57730.13733819405389616No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56950.13548259399565885No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC56030.1332939375167123No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA55310.13158107592449325No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC55220.13136696822546587No Hit
CATCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG54730.1302012707529835No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT53190.12653765012518164No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53090.12629975268181787No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTGTGGTTTTCGGCGGAGG52630.1252054244423446No Hit
GTACTTGATGAAGCTTTACAGTGATGGCGGCCACATCAAGGGGGACGGGA52620.12518163469800822No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG52570.12506268597632636No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC52000.12370667054915294No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCACTAGTAGTTACTACTGGG51680.12294539873038891No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA50920.12113737816082439No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA50460.12004304992135109No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGTAGC48620.115665736963458No Hit
GTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGAACT48590.11559436773044887No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT47900.11395287537123897No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG47850.11383392664955708No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG46420.11043199320945538No Hit
GGATTGGGACTATCTATTATAGTGGGAGTACCTACTACAACCCGTCCCAA46330.11021788551042801No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA45470.1081719674974997No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTGTGGTTTTCGGCGGAGGGA45260.10767238286643582No Hit
GTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTACTGGG44950.10693490079200817No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATGCCAGTGC44450.10574541357518939No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG44350.10550751613182564No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT44340.10548372638748925No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC44290.10536477766580737No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA44220.10519824945545275No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA44130.10498414175642537No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT43780.10415150070465222No Hit
CAGTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGA43770.10412771096031585No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA43620.10377086479527022No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43530.10355675709624285No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG43250.10289064425482433No Hit
GTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGGAGCTGAGCTCTGTGA43200.10277169553314243No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC42930.10212937243606031No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC42300.10063061854286864No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)