FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005110381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361930
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTACTGTGCGACCCTGGGGCATTGTAATAACACCGACTGCCATTC182310.417957188675655No Hit
GTAATAACACCGACTGCCATTCTCATTATTACGATGTGGACGTCTGGGGC168190.38558619693576013No Hit
GTGTTATTACAATGCCCCAGGGTCGCACAGTAATACATGGCGCTGTCCGG107240.24585447267608604No Hit
GTATTACTGTGCGACCCTGGGGCATTGTAATAACACCGACTGCCATTCTC97670.223914643288636No Hit
GTAATACATGGCGCTGTCCGGGGCCTTCAGACTATTCCACTGCAGGTAGG89250.20461126152872697No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC75970.17416602283851415No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT75520.17313436941904156No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA74880.17166712900023615No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA71780.1645601832216473No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA71310.16348267853908707No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCGATTATAAG70910.16256565327733366No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA68830.15779712191621598No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC64720.14837468735169984No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT64290.1473888851953149No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG64160.14709085198524507No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG63920.14654063682819302No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT63680.14599042167114099No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG63360.14525680146173825No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC62630.14358323035903833No Hit
CATTATTACGATGTGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTC62260.14273498199191642No Hit
TATATGAGCTGCAGAAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAG61600.1412218903100233No Hit
ATTATAAGTATGTCTCTTGGTACCAACAACACCCCGGCAAAGCCCCCAAA59020.1353070773717139No Hit
GCCTTGGGCTGACCAAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCT58610.13436712647841667No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57590.1320287120609455No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC57530.1318911582716825No Hit
TCTCCAAGGAGTCTGTGCCGAAGCGCAGCTGGTGCAGTCCGGAGCAGAGG57520.13186823264013864No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC56040.1284752391716511No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC55660.12760406517298534No Hit
CTCCTGCACTGGAACCAGCGATTATAAGTATGTCTCTTGGTACCAACAAC53980.12375255907362108No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT53330.1222623930232718No Hit
GTCCGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAGGATTTCCTGTA52360.12003860676351981No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52350.12001568113197598No Hit
GCTCATATACGAGTAACAGCAGGGTTTTCGGCGGAGGGACCAAGGTGACC51980.11916743276485409No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG51830.11882354829169657No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC50960.11682901834738292No Hit
GTATGTCTCTTGGTACCAACAACACCCCGGCAAAGCCCCCAAACTCATGA50700.1162329519272432No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT50630.11607247250643637No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTTCTGCAGCTCATAT50480.11572858803327885No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG50420.11559103424401583No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA49960.11453645519299945No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA49100.11256485088022962No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT48800.11187708193391459No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA47210.1082319065184448No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG47040.10784217078219963No Hit
GGATACAGCTTTACCAGTTACTGGATCAACTGGGTGCGCCAGATTCCCGG46720.1071085505727969No Hit
TTATTACGATGTGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTCCT46590.10681051736272705No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA46330.10621445094258733No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG45940.10532035131237778No Hit
GTAATAATGAGAATGGCAGTCGGTGTTATTACAATGCCCCAGGGTCGCAC45680.10472428489223806No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA45450.10419699536672986No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA44690.10245464736939841No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)