FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4361930
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTACTGTGCGACCCTGGGGCATTGTAATAACACCGACTGCCATTC158050.36233960655031144No Hit
GTAATAACACCGACTGCCATTCTCATTATTACGATGTGGACGTCTGGGGC141070.3234118841888797No Hit
GTAATACATGGCGCTGTCCGGGGCCTTCAGACTATTCCACTGCAGGTAGG120810.27696455468107006No Hit
GTGTTATTACAATGCCCCAGGGTCGCACAGTAATACATGGCGCTGTCCGG118360.2713477749528305No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT96440.2210947906087443No Hit
GTATTACTGTGCGACCCTGGGGCATTGTAATAACACCGACTGCCATTCTC83940.1924377511789506No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA83850.1922314204950561No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA80050.1835196805083988No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC76150.17457868420630318No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC71660.16428507564312128No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC70900.16254272764578984No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC68860.1578658988108475No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT67900.15566503818263933No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA67000.1536017313436942No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCGATTATAAG65780.15080480429534632No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT64600.1480995797731738No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT63900.14649478556510537No Hit
GCCTTGGGCTGACCAAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCT63380.14530265272482595No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC62940.1442939249368972No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC58810.13482563910929335No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC57990.1329457373226989No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57480.1317765301139633No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT55920.12820013159312507No Hit
TCTCCAAGGAGTCTGTGCCGAAGCGCAGCTGGTGCAGTCCGGAGCAGAGG55380.12696214748975795No Hit
TATATGAGCTGCAGAAATAGTCAGCCTCGTCCTCAGCCTGGAGCCCAGAG55290.12675581680586345No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA54840.1257241633863909No Hit
ATTATAAGTATGTCTCTTGGTACCAACAACACCCCGGCAAAGCCCCCAAA54040.12389011286288408No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT53500.122652128759517No Hit
CTCCTGCACTGGAACCAGCGATTATAAGTATGTCTCTTGGTACCAACAAC52930.12134536776151841No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA52730.12088685513064171No Hit
CAGTAATACATGGCGCTGTCCGGGGCCTTCAGACTATTCCACTGCAGGTA51800.11875477139706506No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGACTATTTCTGCAGCTCATAT51560.11820455624001303No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG51500.11806700245075002No Hit
CATTATTACGATGTGGACGTCTGGGGCCAAGGGACCACAGTCACCGTCTC48960.11224389203861593No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48740.11173952814465156No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA48730.11171660251310774No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT47610.10914893178019822No Hit
GCTCATATACGAGTAACAGCAGGGTTTTCGGCGGAGGGACCAAGGTGACC47250.10832360904462016No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC47030.10781924515065577No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA46280.10609982278486817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA46170.10584764083788599No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG46130.10575593831171064No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC46000.10545790510164078No Hit
GACCAAGGACGGTCACCTTGGTCCCTCCGCCGAAAACCCTGCTGTTACTC45200.10362385457813399No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG44650.10236294484322307No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC44510.10204198600160938No Hit
GTAATAATGAGAATGGCAGTCGGTGTTATTACAATGCCCCAGGGTCGCAC44500.10201906037006554No Hit
CCTCCTGGCTGTTCTCCAAGGAGTCTGTGCCGAAGCGCAGCTGGTGCAGT44400.1017898040546272No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT44380.10174395279153951No Hit
GTCCGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAGGATTTCCTGTA44290.101537622107645No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT43750.10029963800427794No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)