FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530568
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70591.330460939973764No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA44950.8472052592693115No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA44230.8336348969406372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.7493855641501184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39740.7490086096409885No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35090.6613666862683012No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA34310.6466654604122374No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA32300.6087815322446887No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30390.5727823766227892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30120.5676934907495363No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26590.5011610198881199No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26430.4981453838150812No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA23880.4500836839010269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22660.42708945884410665No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA21980.4142730055336922No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA21270.4008911204595829Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG21130.398252438895674No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA20790.39184421224046684No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA17720.3339816950890367No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA17030.32097676452405727No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA16470.31042203826842174No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16440.309856606504727No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC15810.2979825394671371Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA14910.2810195865562944No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA14800.27894633675608027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT14560.2744228826465222No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA14360.2706533375552238No Hit
GTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12890.24294718113418073No Hit
TATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12800.24125088584309645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12490.23540809095158396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12400.23371179566049968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12310.23201550036941543No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC12210.23013072782376623Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA11830.22296859215029932No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11790.22221468313203963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11730.22108381960465012No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11560.21787970627704648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11510.2169373200042219No Hit
GAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11360.21411016118574813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10920.20581716198489167No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA10750.20261304865728802Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10690.20148218512989852No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA9660.18206902790971186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9560.18018425536406268No Hit
GTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9300.17528384674537476No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCA8890.16755627930821307Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8600.16209043892583042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8490.1600171891256163No Hit
GGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA8410.15850937108909696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT8340.15719003030714254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT8020.1511587581610651No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.1511587581610651No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7870.14833159934259132No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7810.14720073581520182No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG7610.14343119072390342No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA7610.14343119072390342No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG7510.14154641817825425Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG7210.13589210054130668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT7000.13193407819544337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT7000.13193407819544337No Hit
GTACGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6920.13042626015892403No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA6890.12986082839522925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT6660.12552585154023613No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6640.1251488970311063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT6540.1232641244854571No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCG6500.12251021546719743Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA6430.12119087468524298No Hit
GTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6360.11987153390328854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.11723285233937969No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGAT5740.10818594412026357Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5690.10724355784743897No Hit
GTATCAACGCAGAGTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAA5640.10630117157461438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5390.1015892402104914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)