FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5641679
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC165780.29384869291570825No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT155790.2761411983914718No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC122330.21683261312811308No Hit
TCTCTGGAGTGCCACATAGGTTCAATGGCAGCGGCTCAGTGACGGATTTC114540.2030246669475523No Hit
ATTTATCACCATGGGCAGACCTACTACAATCCGTCCCTCAAGAGTCGAGT109990.1949596919640412No Hit
CTCCTACTCTATGAGGTTTCTAAGCGCTTCTCTGGAGTGCCACATAGGTT88140.1562300868234439No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCT86840.15392580825672642No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA86480.1532877003459431No Hit
GTCTTGGCTCGTGCAGAGGTCAGGCCAGCCTCCACAACTCCTACTCTATG86240.1528622950720876No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT85350.15128475051487333No Hit
ACTACAATCCGTCCCTCAAGAGTCGAGTCTCCATATCAGTCGACACGTCT80380.14247531630211505No Hit
CTCCATATCAGTCGACACGTCTAGGAGCCAGATTTCCCTGGCTTTACATA79490.14089777174490076No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC75630.1340558369237243No Hit
CCCCTGGACAGCCGGCCTCCATTTCCTGCAAGTCTAGTCAGAGCCTCCTC75430.1337013325288447No Hit
ACCTACTACAATCCGTCCCTCAAGAGTCGAGTCTCCATATCAGTCGACAC74930.13281507154164568No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT74100.1313438783028953No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT73980.13113117566596755No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT71510.12675304638920434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC71140.12609721325867707No Hit
ATGTAAAGCCAGGGAAATCTGGCTCCTAGACGTGTCGACTGATATGGAGA70960.12577815930328543No Hit
TACTATGCCTATTCGTGGTACTTTGACTACTGGAGCCCGGGAACCCTAGT68230.12093917431317877No Hit
GTGCAGAGGTTGTCTTGACCCAGACTCCACTCCATCTGTCCGTCACCCCT65070.11533800487408093No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG63710.11292737498889958No Hit
GATATGGAGACTCGACTCTTGAGGGACGGATTGTAGTAGGTCTGCCCATG63670.11285647410992365No Hit
TTACTATGCTTATGGTCCTTACTATGCCTATTCGTGGTACTTTGACTACT63490.11253742015453201No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC62800.11131437999219736No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG61550.10909872752419979No Hit
TTTCTACTGGGGGTGGGTCCGCCAGTCCCCCGGCAAGGGCCTGGAATGGA61050.10821246653700078No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG60490.10721985423133788No Hit
GTAGTAGGTCTGCCCATGGTGATAAATAGTTCCAATCCATTCCAGGCCCT60050.10643994456260274No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT59620.10567776011361157No Hit
ATGTTGGGACTTATCACTGCATGCAAGGCTCACATCTTCCGTGGACGTTC59600.10564230967412361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT59200.10493330088436438No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG57320.10160095957249607No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)