FastQCFastQC Report
Mon 8 Aug 2022
EGAF00005110406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5641679
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT182500.32348526032764363No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC141220.2503155532244922No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT121670.2156627486250104No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC102700.18203800677067944No Hit
ATTTATCACCATGGGCAGACCTACTACAATCCGTCCCTCAAGAGTCGAGT95880.16994940690528476No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT89180.15807350967681782No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA82830.14681799513939026No Hit
TCTCTGGAGTGCCACATAGGTTCAATGGCAGCGGCTCAGTGACGGATTTC73090.12955363110875326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG72460.12843694226488248No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC71120.12606176281918913No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA70060.1241828895263272No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG69240.12272942150732077No Hit
CTCCATATCAGTCGACACGTCTAGGAGCCAGATTTCCCTGGCTTTACATA68810.12196723705832963No Hit
ACTACAATCCGTCCCTCAAGAGTCGAGTCTCCATATCAGTCGACACGTCT67390.1194502558546844No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA67080.118900774042621No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT66080.11712825206822296No Hit
ACCTACTACAATCCGTCCCTCAAGAGTCGAGTCTCCATATCAGTCGACAC65080.11535573009382491No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT64160.11372500987737869No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC63620.11276784801120376No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT61870.10966593455600718No Hit
ATGTAAAGCCAGGGAAATCTGGCTCCTAGACGTGTCGACTGATATGGAGA61420.10886829966752806No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT60390.10704260203389807No Hit
ATGTTGGGACTTATCACTGCATGCAAGGCTCACATCTTCCGTGGACGTTC60200.10670582285876244No Hit
TTTCTACTGGGGGTGGGTCCGCCAGTCCCCCGGCAAGGGCCTGGAATGGA59030.10463197214871672No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA58960.10450789561050886No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58770.10417111643537323No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)