FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825999
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58590.7093228926427272No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA35560.43050899577360263Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.4205816229801731No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA33450.4049641706588022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.3969738462153102No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA26630.32239748474271757No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA26470.32046043639278016No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24820.30048462528404996No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG24700.2990318390215969Clontech Universal Primer Mix Long (96% over 25bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC23740.287409548921972Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21760.26343857559149586No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC20360.24648940252954302Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19360.23438285034243383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG18650.2257871982895863No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA16720.20242155256846558Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16620.20121089734975467No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA16570.20060556974039917No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA15920.19273631081877823No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA15440.18692516576896584No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA15000.18159828280663778No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14630.17711885849740738No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14370.173971154928759No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA14310.17324476179753243Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCA13560.16416484765720055Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA12790.15484280247312648No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC12300.14891059190144298Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA12210.14782100220460315No Hit
TATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11980.14503649520156806No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG11890.14394690550472822Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA11190.13547231897375178No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10740.13002437048955265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10650.12893478079271284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10370.12554494618032225No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCG10280.12445535648348244Clontech Universal Primer Mix Long (96% over 26bp)
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC10090.12215511156793168Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9890.11973380113050984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT9880.11961273560863875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9630.11658609756186146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9470.114649049211924No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9460.11452798369005288No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGAT9170.11101708355579123Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA9160.11089601803392014No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT9030.10932216624959595Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC8850.1071429868559163Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA8730.1056902005934632Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT8370.10133184180610387No Hit
GGTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA8290.10036331763113515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)