FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93724
Sequences flagged as poor quality0
Sequence length101
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT8780.9367931372967436No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGT8230.878110195894328No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT7780.8300968802014426No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGT7410.7906192650761812No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGG6810.7266015108190005No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGG6460.6892578208356451No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG5410.577226750885579No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGG5190.5537535743246127No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGG4630.4940036703512441No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGT4520.48226708207076097No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGG3880.4139814775297682No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGT3820.40757970210405015No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGT3770.4022448892492851No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGT3530.37663778754641286No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGG3260.3478297981306816No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGT3250.34676283555972853No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGGCGT3030.32328965899876233No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGG2960.31582092100209125No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT2800.29874951986684306No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGG2480.2646067175963467No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTGTGCCGG2380.2539370918868166No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGGCGT2320.24753531646109855No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGT2290.24433442874823952No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT2280.2432674661772865No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGG2120.22619606504203832No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGTCTT2100.22406213990013232No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGTCGT2090.2229951773291793No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGGCTT2010.2144594767615552No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG2000.2133925141906022No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGG2000.2133925141906022No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGTCTT1890.20165592591011908No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGG1870.19952200076821303No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGT1860.19845503819726004No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGGCGT1840.19632111305535402No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGGCGT1810.19312022534249498No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGTCGT1680.17924971192010583No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGT1670.17818274934915285No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGT1660.1771157867781998No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGGCGT1640.17498186163629378No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGG1580.16858008621057574No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGGTGCCGG1520.16217831078485767No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGGCGT1510.16111134821390466No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGGTGCCGG1500.16004438564295165No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGG1480.15791046050104562No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGGCTT1450.1547095727881866No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGGCTT1430.15257564764628057No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGG1420.15150868507532755No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTTCCGT1410.15044172250437454No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTGTGCCGT1380.1472408347915155No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTTCCGG1360.14510690964960948No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGGCGG1260.1344372839400794No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTGTGCCGG1250.13337032136912638No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTGCGT1210.12910247108531434No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGTCGT1200.12803550851436132No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGTCGT1190.1269685459434083No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGGCTT1170.12483462080150227No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGGTGCCGGCGT1150.12270069565959627No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATTCCGT1150.12270069565959627No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT1140.12163373308864325RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGGCGT1140.12163373308864325No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGGCGG1130.12056677051769024No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGGCGT1130.12056677051769024No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGGCGT1110.11843284537578422No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGGCGT1110.11843284537578422No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATTCCGG1090.1162989202338782No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGGCTT1080.11523195766292518No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGTCTT1070.11416499509197217No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGGTGCCGT1070.11416499509197217No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGTCTT1060.11309803252101916No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGGCGT1030.10989714480816014No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGTCGT1020.10883018223720711No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGTCTT1000.1066962570953011No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGTCGT990.10562929452434808No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTTCCGG980.10456233195339508No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGTCTT980.10456233195339508No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGGGGT980.10456233195339508No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCTT970.10349536938244205No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGGCTT940.10029448166958303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)