FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005110453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177599
Sequences flagged as poor quality0
Sequence length101
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT16390.9228655566754318No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGT16200.912167298239292No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT14740.8299596281510594No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGT13680.7702746074020687No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGG13540.762391680133334No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGG11530.6492153672036441No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGG10650.5996655386573122No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG9580.5394174516748406No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGG7690.43299793354692306No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGT7260.4087860855072382No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGT7150.4025923569389468No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGG6930.3902048998023638No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGT6880.38738956863495855No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGGCGT6560.3693714491635651No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGG6130.34515960112388017No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGT6120.34459653489039915No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGGCGT5620.31644322321634694No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT5330.30011430244539666No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGT5170.29110524270969995No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGG5130.2888529777757758No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG4660.26238886480216667No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGG4540.2556320700003942No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGG4420.24887527519862163No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGTCTT4390.24718607649817848No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGGCTT4240.2387400829959628No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGTCTT4200.2364878180620386No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGT4070.22916795702678508No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGTCGT3990.22466342715893672No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT3990.22466342715893672No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGGCGT3960.22297422845849357No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGG3910.22015889729108834No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGGCGT3890.21903276482412626No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTGTGCCGG3880.21846969859064522No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGT3850.21678049989020207No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGTCGT3620.20382997652013804No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGGCTT3360.18919025444963092No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGGCTT3160.17792892978001001No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGGCGT3130.1762397310795669No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGT3090.17398746614564273No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGGTGCCGG3080.17342439991216166No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGGCGT3050.17173520121171854No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGG3040.1711721349782375No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGT2960.16666760511038914No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGGTGCCGG2960.16666760511038914No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGGTGCCGGCGT2910.1638522739429839No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTGCGT2860.1610369427755787No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT2770.1559693466742493RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGTCGT2610.14696028693855256No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGG2600.14639722070507152No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGG2600.14639722070507152No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTTCCGG2590.14583415447159048No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGGCTT2560.14414495577114736No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTTCCGT2550.14358188953766632No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGTCTT2540.14301882330418528No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGGCGT2530.14245575707070424No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGTCTT2510.14132962460374215No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGTCGT2460.13851429343633692No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGG2420.13626202850241273No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTGTGCCGT2420.13626202850241273No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTGTGCCGG2410.1356989622689317No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGGCGT2330.13119443240108333No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGGCGG2280.12837910123367813No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGGCGT2270.1278160350001971No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGTCTT2270.1278160350001971No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCGT2250.12668990253323498No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGGCTT2220.12500070383279185No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGTCGT2200.12387457136582977No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATTCCGG2130.11993310773146244No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGTCTT2100.1182439090310193No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGGCTT2050.11542857786361409No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGTCTT2010.11317631292968992No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATTCCGT2000.11261324669620887No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGGCTT1980.11148711422924679No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCGT1980.11148711422924679No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGGCGG1930.10867178306184157No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGGCGT1900.10698258436139844No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGTCTT1860.10473031942747427No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGGGGT1820.10247805449355007No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGGTGCCGT1810.10191498826006902No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGGCGT1800.10135192202658798No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)