FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7065209
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA260380.368538283863931No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT151900.21499717842741808No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC143240.20273993310035132No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT135330.19154422749560557No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT129560.18337744856521582No Hit
TATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTG123690.1750691310051833No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT123180.17434728399400498No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT121030.1713042034566847No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATC119930.1697472785306139No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT103940.14711525165072964No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA102980.14575648080615874No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA102120.14453924859123063No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC98060.13879278022773284No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA94400.13361246638280624No Hit
GTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCG93940.13296138868644933No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTG92710.13122046354184286No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG91880.1300456929158076No Hit
CCTGAAAGAGGTATGCTCTCTTAGGAATACAGTTTCTAGCATTAAACAAA88780.1256579953968807No Hit
TATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAA88430.12516261019313088No Hit
ATGTTTATGCATATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAA86930.12303953074848882No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGA84570.11969921908891866No Hit
TTCTTACACTAACCTGATTATGAAAAAAAGAAGTCTGTATCATCTGCTTC83320.11792998621838362No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC82240.11640136901824136No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAAT81200.11492936726995621No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAA81160.11487275181809907No Hit
CTTCTTAATTATATGTTCATACTCTAAGTATCAAAATCTTCCAATTATCA81130.11483029022920624No Hit
GTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAAA76310.10800812828042312No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT72790.10302596851699646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72080.10202104424653256No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC70880.10032258069081891No Hit
ATTAAGAAGTGATTACCTAATAAAGATAACAATGTGACTATTTTAATTAT70730.10011027274635471No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)