FastQCFastQC Report
Mon 25 Jul 2022
EGAF00005110457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106448
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGT7560.7102059221403878No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGT7460.7008116639110176No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT7350.6904779798587103No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGG6540.6143844882008117No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGT6430.6040508041485044No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGG5540.5204419059071096No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGG4960.4659552081767624No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG4810.45186382083270704No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGG4630.43495415601984067No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGT3780.3551029610701939No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGT3760.35322410942431987No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGATGCCGG3570.33537501878851644No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGT3260.3062528182774688No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGT3170.2977979858710356No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGG2730.25646324966180667No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGT2630.2470689914324365No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGGCGT2550.23955358484894032No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGG2540.2386141590260033No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGGCGT2530.23767473320306629No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGT2350.2207650683901999No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGG2210.20761310686908163No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTATGCCGG2090.19633999699383736No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG2070.19446114534796333No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGTCTT1960.1841274612956561No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGT1930.18130918382684502No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGG1920.180369758003908No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTGTGCCGG1870.17567262888922291No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGT1790.16815722230572674No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGTCGT1760.1653389448369157No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGT1760.1653389448369157No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGT1760.1653389448369157No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGTCTT1750.16439951901397867No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGGTGCCGG1700.15970238989929356No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGCCGGCTT1680.15782353825341952No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGGCGG1630.1531264091387344No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTCCGG1610.15124755749286037No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGGTGCCGG1610.15124755749286037No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGGCGT1590.1493687058469863No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGGCGT1530.1437321509093642No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTGTGCCGG1500.14091387344055312No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGGCGGCGT1450.13621674432586803No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGGCTT1450.13621674432586803No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGT1440.135277318502931No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTGTGCCGT1440.135277318502931No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT1410.13245904103411996RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGCCGTCGT1410.13245904103411996No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGGCGG1360.12776191191943484No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGGCGT1320.12400420862768675No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTTCCGG1250.11742822786712762No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATTCCGG1240.1164888020441906No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTTGCGT1230.11554937622125358No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGGCTT1170.10991282128363146No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGTTGCCGTCGT1140.10709454381482036No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGTTTGCCGGCGT1130.10615511799188335No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTTCCGT1110.10427626634600931No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTTTGGCGTCTT1090.10239741470013528No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGTTGGCGTCGT1090.10239741470013528No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCGCGGGTGCCGGCGT1080.10145798887719824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)