FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56964
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT690912.128712871287128No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT50198.81082789129977TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT30345.3261709149638365No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTT28725.0417807738220635No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT27534.832876904711748RNA PCR Primer, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCT15962.8017695386559933Illumina PCR Primer Index 9 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT13462.3628958640544906TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG11852.0802612176111226TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCTTG7361.292044098026824Illumina PCR Primer Index 9 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCT7151.2551787093602977Illumina PCR Primer Index 9 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG5640.9900990099009901TruSeq Adapter, Index 11 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC5470.960255600028088No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA5040.8847693279966296RNA PCR Primer, Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT4620.8110385506635769No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTG3610.6337335861245699Illumina PCR Primer Index 9 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTC3160.5547363246962994No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC2570.4511621374903448RNA PCR Primer, Index 17 (95% over 24bp)
CTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCTTGA2440.4283407064110667Illumina PCR Primer Index 9 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCT2330.4090302647286005No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGC1380.2422582683800295Illumina PCR Primer Index 9 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA1180.20714837441190925No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCG840.1474615546661049RNA PCR Primer, Index 11 (95% over 22bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT790.13868408117407485TruSeq Adapter, Index 11 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCCTAGATCTCGTATGCCGT650.11410715539639071No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCG600.10532968190436064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)