FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64625
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT926214.33191489361702No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT728011.264990328820117TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT37175.751644100580271RNA PCR Primer, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT20903.234042553191489No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTT20893.232495164410058No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG19072.950870406189555TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT19022.943133462282398TruSeq Adapter, Index 11 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCT10081.5597678916827853Illumina PCR Primer Index 9 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG8951.3849129593810445TruSeq Adapter, Index 11 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC8091.2518375241779498No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA7881.2193423597678916RNA PCR Primer, Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT6410.9918762088974855No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCTTG4910.7597678916827854Illumina PCR Primer Index 9 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCT4830.7473887814313346Illumina PCR Primer Index 9 (95% over 24bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC3850.5957446808510638RNA PCR Primer, Index 17 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTG2700.4177949709864603Illumina PCR Primer Index 9 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTC2210.34197292069632496No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCT1840.28471953578336556No Hit
CTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCTTGA1800.27852998065764023Illumina PCR Primer Index 9 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCG1260.19497098646034816RNA PCR Primer, Index 11 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA1250.19342359767891684No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT1240.1918762088974855TruSeq Adapter, Index 11 (96% over 27bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGC790.12224371373307545Illumina PCR Primer Index 9 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGTCCTAGATCTCGTATGCCGT680.10522243713733076No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCGT680.10522243713733076No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG660.10212765957446808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)