FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172385
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA117300.36975335591361075No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT89870.2832884407157391No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT66540.20974755586096894TruSeq Adapter, Index 11 (95% over 21bp)
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT66490.20958994573483358No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT63650.20063769057034378No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT59490.18752452807587983No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT59140.18642125719293212No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC58960.18585386073884475No Hit
TATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTG57720.18194512961068723No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATC56000.17652334127163No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG49630.15644381120198209No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA46180.14556871249864062No Hit
TTCTTACACTAACCTGATTATGAAAAAAAGAAGTCTGTATCATCTGCTTC45880.1446230517418283No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT45470.14333064870751816No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGA44140.13913821935231696No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA42420.13371643101325975No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC42070.13261316013031205No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA41870.13198271962577052No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT41210.12990226596078344No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTG40860.12879899507783577No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTT40410.1273805039426173No Hit
GTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCG40070.1263087550848967No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAAT39890.12574135863080932No Hit
CCTGAAAGAGGTATGCTCTCTTAGGAATACAGTTTCTAGCATTAAACAAA39820.12552070445421978No Hit
GTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAAA39690.1251109181262678No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT39520.12457504369740746No Hit
CGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATG39430.12429134547036379No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT38860.12249459003242039RNA PCR Primer, Index 11 (95% over 23bp)
TATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAA38040.11990978396380012No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC37440.1180184624501755No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT35310.11130427107680814No Hit
GTGTAATAAAATGTTCCTCGCATTCCCCCCCGCTTTTTTTTTTTTTTTTT34930.11010643411817922No Hit
AATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTGAACAA34670.10928686146227523No Hit
GGTCAGACAAATGGTACACAAATCTGGTTCTCAATGGTGAGGTGGGATCA34590.10903468526045862No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC34290.10808902450364631No Hit
GGTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAA34250.107962936402738No Hit
ATGTTTATGCATATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAA34220.10786837032705676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)