FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172385
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450431.4198465823032198No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA120850.380943674869223No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC64600.20363228296691607No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT63980.2016779174028373No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT63590.20044855841898132No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT58730.18512885415862199No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT58570.18462450175498876No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC56750.1788874931636608No Hit
TATTAAGGCTGTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTG53650.16911566534326697No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATC52370.16508084611420115No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG51230.16148733523831438No Hit
TTCTTACACTAACCTGATTATGAAAAAAAGAAGTCTGTATCATCTGCTTC48470.1527872562756412No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA47290.14906765729884613No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT44550.1404306223866271No Hit
CTCGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGA43940.13850777884777543No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA41610.1311631469698665No Hit
TGTCTGACCTCTGTAAAAAATGTGATCCTACAGAAGTGGAGCTGGATAAT41530.1309109707680499No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT41150.12971313380942098No Hit
ATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATTGTTCCTCTG41030.12933486950669607No Hit
GTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCG40840.1287359510273816No Hit
CCTGAAAGAGGTATGCTCTCTTAGGAATACAGTTTCTAGCATTAAACAAA40550.12782181229579637No Hit
CGTATATGGTGGTGAGACCAAAATGGTGGAAACAGCCTTAACCCCAGATG40020.1261511449587613No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA37420.11795541839972135No Hit
GTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAAA36810.11603257486086965No Hit
TATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAA36730.11578039865905304No Hit
TTTCTGGGGAGTCCTGGCGGTTTTTATTAAGGCTGTTCATGTGAAAGCCC35670.1124390639849829No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT35090.11061078652181246No Hit
GGTCAGACAAATGGTACACAAATCTGGTTCTCAATGGTGAGGTGGGATCA35040.11045317639567706No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.10276180224027033No Hit
ATGTTTATGCATATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAA32560.10263571413936202No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC31790.10020851819687712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)