FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746262
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT140490.3750138137695655No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT95960.25614866231993383TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT56910.15191142530874777RNA PCR Primer, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT56150.14988273644502173No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTT52300.13960582575377803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)