FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107265
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT1285411.983405584300565No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1092210.182258891530322TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTT65626.11755931571342No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT57055.318603458723722No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT57055.318603458723722RNA PCR Primer, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCT33493.1221740549107353Illumina PCR Primer Index 9 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT31592.9450426513774297TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG30982.8881741481377894TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCTTG18451.7200391553628864Illumina PCR Primer Index 9 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCT17641.6445252412250038Illumina PCR Primer Index 9 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG13411.2501748007271711TruSeq Adapter, Index 11 (95% over 24bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA12681.1821190509485853RNA PCR Primer, Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC10720.999394024145807No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT8990.8381112198760081No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTG8320.775649093366895Illumina PCR Primer Index 9 (95% over 22bp)
CTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGCTTGA6750.6292826178156902Illumina PCR Primer Index 9 (96% over 26bp)
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC6400.5966531487437654RNA PCR Primer, Index 17 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTC6220.5798722789353471No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCT5040.4698643546357153No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCTGC3230.30112338600661914Illumina PCR Primer Index 9 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA2680.24984850603645176No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCG2080.19391227334172376RNA PCR Primer, Index 11 (95% over 22bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT2000.1864541089824267TruSeq Adapter, Index 11 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCTTCG1280.11933062974875308No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGTCGT1160.10814338320980749No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTCCTAGATCTCGTATGCCGT1130.10534657157507109No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)