FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114267
Sequences flagged as poor quality0
Sequence length101
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1638914.342723620992937No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT111639.769224710546352RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT58575.125714335722475RNA PCR Primer, Index 17 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT57335.017196565937673No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT56044.9043030796292895No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT31262.735697970542676RNA PCR Primer, Index 17 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCT28292.4757804090419806RNA PCR Primer, Index 19 (95% over 21bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG25782.2561194395582276RNA PCR Primer, Index 17 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCT15141.324966963340247RNA PCR Primer, Index 19 (95% over 24bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG13511.182318604671515TruSeq Adapter, Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC12761.116682856817804No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTG12571.1000551340281972RNA PCR Primer, Index 19 (96% over 26bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA10210.8935213141151863RNA PCR Primer, Index 17 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT9870.8637664417548374No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTG6190.5417137056192952RNA PCR Primer, Index 13 (95% over 22bp)
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC6050.5294616993532691TruSeq Adapter, Index 25 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTC6030.5277114127438368No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTGA4840.4235693594826153RNA PCR Primer, Index 19 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCT4380.3833127674656725No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGC2710.2371638355780759RNA PCR Primer, Index 13 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCG2520.2205361127884691RNA PCR Primer, Index 17 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA2100.18378009399039094No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT1810.15840093815362266TruSeq Adapter, Index 12 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCG1550.13564721223100282No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCGT1340.11726920283196374No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG1240.10851776978480225No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCGT1200.10501719656593768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)