FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118254900
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG504450.019.5357079
GAGCGTC621950.016.0644268
AGAGCGT706900.014.2968617
CGCCGTA187600.013.90002155-59
ATCGGAA745050.013.2497231
TCGGAAG769350.012.8357762
TCGCCGT215300.012.25848555-59
CGGAAGA854850.011.7476763
AAGAGCG862700.011.6910166
GCCGTAT243550.010.978168555-59
GTCGCCG241600.010.93000155-59
TGGTCGC296350.09.14290150-54
GTGGTCG311550.08.83978450-54
CGTATCA311900.08.719542560-64
CCGTATC291800.07.168820455-59
TCTCGGT401400.07.053663745-49
GGTCGCC297850.07.042105750-54
GACGTGT403700.06.81768735-39
GAAGAGC1642600.06.64741375
CGGTGGT449350.06.339941550-54
Produced by FastQC (version 0.11.5)