FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117253
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1541113.143373730309671No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1256910.719555149974841RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT73096.233529206075751RNA PCR Primer, Index 17 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT64065.46339965715163No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT55644.745294363470444No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT42303.607583601272462RNA PCR Primer, Index 17 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCT35142.996938244650457RNA PCR Primer, Index 19 (95% over 21bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG34142.9116525803177744RNA PCR Primer, Index 17 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCT20621.7585903985399096RNA PCR Primer, Index 19 (95% over 24bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG17111.4592377167321944TruSeq Adapter, Index 12 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTG17081.456679146802214RNA PCR Primer, Index 19 (96% over 26bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA13521.153062181777865RNA PCR Primer, Index 17 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC13291.133446478981348No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT10890.9287608845829104No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTG9050.771835262210775RNA PCR Primer, Index 13 (95% over 22bp)
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC8870.7564838426308922TruSeq Adapter, Index 25 (95% over 24bp)
CTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTGA6650.5671496678123374RNA PCR Primer, Index 19 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTC6100.5202425524293621No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCT4190.35734693355393893No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGC3740.3189683846042319RNA PCR Primer, Index 13 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA3160.2695026992912761No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCG2220.18933417481855475RNA PCR Primer, Index 17 (95% over 22bp)
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT2120.1808056083852865TruSeq Adapter, Index 12 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCGT1490.12707563985569664No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCGT1300.11087136363248701No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)