FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58353993
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA427450.016.85821
TCGGAAG444000.016.3276142
CGGAAGA458050.015.7942283
TATGCCG133500.013.8247945-49
CTCGTAT151400.012.0945140-44
CGTATGC152750.011.70289345-49
TCTCGTA150200.011.06179240-44
CGGCGAC36850.010.7096641
ATGCCGT172050.010.70188945-49
GCCGTCT176300.010.02534650-54
AGAGCAC767650.09.8773547
AGCACAC799200.09.5599879
GAGCACA820450.09.2858748
CGAGCAA200750.09.27304130-34
ATCTCGT181250.09.19078940-44
TCGTATG168800.09.04412940-44
GAAGAGC855500.08.9225455
GTCACGA210850.08.8019525-29
ACGAGCA213950.08.70770130-34
CGTCTTC213850.08.70570150-54
Produced by FastQC (version 0.11.5)