FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58353993
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG306900.023.1737529
GAGCGTC368850.019.4780858
AGAGCGT407850.017.7401667
ATCGGAA442950.016.4043851
TCGGAAG458000.015.9410622
CGCCGTA111500.015.87976855-59
AAGAGCG482150.015.1265956
CGGAAGA483750.015.1061223
TCGCCGT126400.014.06522755-59
GCCGTAT140600.012.70658255-59
GTCGCCG150500.011.93818155-59
TGGTCGC167400.010.88026750-54
CGTATCA170750.010.67465560-64
GTGGTCG177150.010.26506650-54
GTCGATC34450.09.6801483
CCGTATC161100.09.37040855-59
GAAGAGC885750.08.7414895
GGTCGCC180450.08.34956850-54
TGCTCGT218650.08.32906235-39
GCTCGTG221250.08.27050435-39
Produced by FastQC (version 0.11.5)