FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6639392
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT200490.3019704213879825No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT134660.2028197762686704RNA PCR Primer, Index 17 (95% over 21bp)
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT111280.16760570847451092No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC91020.13709086615159943No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT80790.12168282878914213RNA PCR Primer, Index 17 (95% over 23bp)
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC77340.1164865698545891No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT72970.10990464187082191No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT68640.10338296036745533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)