FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3986115
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT309120.7754919263493402No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT205040.5143855608781984RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT111630.28004711354288575RNA PCR Primer, Index 17 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT104130.26123180088883535No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT99680.2500680487140988No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT54280.13617268944824723RNA PCR Primer, Index 17 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCT51090.12816990979939114RNA PCR Primer, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)