FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64482
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1112517.25287677181229No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT737211.432647870723613RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT41516.43745541391396RNA PCR Primer, Index 17 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGT37235.773704289569182No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTT34705.38134673242145No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT22163.4366179709066094RNA PCR Primer, Index 17 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCT18832.9201947830402286RNA PCR Primer, Index 19 (95% over 21bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG16822.60847988585962RNA PCR Primer, Index 17 (96% over 28bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCT10491.6268105827983004RNA PCR Primer, Index 19 (95% over 24bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG9841.526007257839397TruSeq Adapter, Index 12 (95% over 23bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTG8181.268571074098198RNA PCR Primer, Index 19 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC8011.2422071275704847No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT6260.9708135603734375No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA5770.8948233615582644RNA PCR Primer, Index 17 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTG4800.744393784311901RNA PCR Primer, Index 13 (95% over 22bp)
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC4060.6296330758971496TruSeq Adapter, Index 25 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTC3510.5443379547780777No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCT2720.4218231444434106No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGCTTGA2150.33342638255637236RNA PCR Primer, Index 19 (96% over 26bp)
ACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCTGC2010.3117148971806085RNA PCR Primer, Index 13 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAGGCAGA1730.268291926429081No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCG1680.26053782450916535RNA PCR Primer, Index 17 (95% over 22bp)
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT1210.18764926646195837TruSeq Adapter, Index 12 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCG820.12716727148661644No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCGT750.11631152879873453No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG750.11631152879873453No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCGT690.10700660649483576No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCGCGTATGCCGTCTTCGGCT670.10390496572686952No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)