FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77105586
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGCATATCTCGTATG788880.10231165352922679TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA757950.018.089091
TCGGAAG763900.017.8489172
CGGAAGA791550.017.0880853
TATGCCG225000.014.95633145-49
CTCGTAT257750.012.99322440-44
CGTATGC256750.012.84140345-49
ATGCCGT266300.012.5768945-49
TCTCGTA252950.012.56346740-44
AGAGCAC1154600.012.2234487
GCCGTCT272600.011.82863650-54
AGCACAC1206000.011.7502539
GAGCACA1218900.011.5426418
TATCTCG276750.011.28396440-44
GAAGAGC1283150.010.964975
ATCTCGT305300.010.52791940-44
CGTCTTC321450.010.41895850-54
TGCCGTC272600.010.13746745-49
TCGTATG278350.010.13052740-44
CCGTCTT332500.010.04654150-54
AAGAGCA1664250.08.6892776
Produced by FastQC (version 0.11.5)