FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916490
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT136830.46915984625354445No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT117420.40260724363875755RNA PCR Primer, Index 26 (100% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT88250.302589756865273RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT78560.26936488724459884No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT69190.23723722694060323RNA PCR Primer, Index 26 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48780.16725584521119566No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCT47620.16327846143823568RNA PCR Primer, Index 26 (95% over 24bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT41150.14109426056664004RNA PCR Primer, Index 26 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)