FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918470
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT202180.5159666910809576No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT150690.3845633627410699RNA PCR Primer, Index 26 (100% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT107050.2731933637363563RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT106660.2721980773107871No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT87080.22222959471426348RNA PCR Primer, Index 26 (100% over 24bp)
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT67530.17233767261201438No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCT57550.14686854818334708RNA PCR Primer, Index 26 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47990.12247127067452349No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT46270.11808180233611588RNA PCR Primer, Index 26 (100% over 27bp)
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGT43190.1102215915905953No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT42550.10858830104607156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)