FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4911291
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT168760.34361637296588615No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT132070.2689109645508686RNA PCR Primer, Index 26 (100% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT99400.20239077668173194RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT89210.18164266788508357No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT74060.1507953814994876RNA PCR Primer, Index 26 (100% over 24bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCT53250.10842363036521355RNA PCR Primer, Index 26 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)