FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3170406
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT178360.5625777897215688No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT159580.5033424741184568RNA PCR Primer, Index 26 (100% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTT121880.3844302590898453RNA PCR Primer, Index 26 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGT110670.3490720115972529No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT95060.2998354154010559RNA PCR Primer, Index 26 (100% over 24bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCT67180.2118971513427618RNA PCR Primer, Index 26 (95% over 24bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT51610.16278672195296123RNA PCR Primer, Index 26 (100% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG50720.15997951051064122RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCGCGTATGCCGTCTTCTGCT34090.10752566075133595RNA PCR Primer, Index 26 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)