FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64918689
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA407350.022.1718031
TCGGAAG425000.021.3779052
CGGAAGA450100.020.311213
TATGCCG150600.012.48709445-49
AGAGCAC792200.012.0613137
AGCACAC851000.011.2703219
CGTATGC170350.011.17555145-49
CTCGTAT170650.011.08791340-44
GAGCACA870600.010.9751588
GAAGAGC896000.010.6073945
TCTCGTA167200.010.34545940-44
ATGCCGT186550.010.13511645-49
TCGTATG187800.09.95182840-44
TGCCGTC196500.09.44482245-49
GCCGTCT200250.09.18124450-54
ATCTCGT206200.08.50125840-44
AAGAGCA1211200.08.1462226
CGTCTTC237200.08.11779750-54
CCGTCTT242750.07.926226650-54
GGAAGAG1416300.07.06896974
Produced by FastQC (version 0.11.5)