FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89018960
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCTCGTATG1545990.17366974406351185TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA694500.036.3022351
TCGGAAG711650.035.5824432
CGGAAGA749450.033.758753
AGAGCAC1213500.021.3514757
AGCACAC1269200.020.4086579
GAGCACA1304500.019.8453568
TATGCCG307550.019.08957345-49
GAAGAGC1357550.019.0692235
CGACGTC317650.018.18923230-34
ACGACGT321650.017.88640230-34
CTCGTAT339350.017.28719340-44
CGTATGC335100.017.25186245-49
TCTCGTA337050.016.86743240-44
CACGACG337750.016.86207630-34
ATGCCGT359450.016.24453545-49
CGTCATA361000.015.85635435-39
TATCTCG356150.015.70636740-44
GCCGTCT371550.015.37173350-54
ATCTCGT387800.014.66752940-44
AAGAGCA1805600.014.6023986
Produced by FastQC (version 0.11.5)