FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241866
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT105070.46867207941955497No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT80290.3581391572912922RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT48490.21629303446325515No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT45960.20500779261561575RNA PCR Primer, Index 27 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT43950.19604204711610773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.13180984055246836No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCT25890.11548415471754334RNA PCR Primer, Index 38 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)