FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3655083
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT127330.3483641821540031No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT95510.2613073355652936RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT58230.15931238770774836No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT54160.14817720965570413No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT53870.1473837940205462RNA PCR Primer, Index 27 (96% over 25bp)
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA40270.11017533664762197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.1096281534509613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)