FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979797
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT465381.561784242349395No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT323891.0869532387608956RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT189060.6344727509961249No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT189050.6344391916630563No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT160330.5380567870898588RNA PCR Primer, Index 27 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCT93680.3143838321872262RNA PCR Primer, Index 38 (95% over 21bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT72880.24458041940440908RNA PCR Primer, Index 27 (96% over 28bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCTGCT47710.16011157807058668RNA PCR Primer, Index 38 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.11661868241360067No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG34330.11520919042471685RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC33550.11259156244536123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)