FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580733
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT190530.7382786208414431No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT159330.617382735835129RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT97560.37803213273128217RNA PCR Primer, Index 27 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT92740.3593552684450503No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT79210.3069283029278891No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCT56220.21784508509791597RNA PCR Primer, Index 38 (95% over 21bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT47580.18436622463462898RNA PCR Primer, Index 27 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32970.12775440155955692No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCTGCT30200.11702101689713737RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)