FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770687
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT261780.9448198226649203No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT212000.7651531912482356RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT126100.4551217802660495No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT117170.42289150669130077RNA PCR Primer, Index 27 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT109940.3967968954992029No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCT68280.2464370749925921RNA PCR Primer, Index 38 (95% over 21bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT55300.19958948809446897RNA PCR Primer, Index 27 (96% over 28bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCTGCT38320.13830504853128484RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)