FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339752
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT283770.8496738679997796No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT210250.6295377620853285RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTT118930.3561042855876724No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGT113150.33879761131964287No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT109750.32861721469139027RNA PCR Primer, Index 27 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCGCGTATGCCGTCTTCT64420.19288857376236318RNA PCR Primer, Index 38 (95% over 21bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT51900.15540076029597408RNA PCR Primer, Index 27 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)