FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70403422
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGAGCGATCTCGTATG1454010.20652547258285256TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGAGCGATCTCGTAT848370.12050124495368988TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA821550.031.4678781
TCGGAAG827750.031.2227332
CGGAAGA848100.030.4663433
GATCGGA631700.022.0552771
AGAGCAC1202500.021.8300447
TATGCCG367700.021.79016145-49
AGCACAC1229300.021.3659239
GAGCACA1253550.021.033568
CTCGTAT392900.020.44421240-44
GAAGAGC1308650.020.0427155
CGTATGC396250.020.00428645-49
TCTCGTA364150.019.9480340-44
ATGCCGT409600.019.39122845-49
GCCGTCT410550.018.83432250-54
GAGCGAT393550.018.20382535-39
ATCTCGT403650.017.93131840-44
CGTCTTC456400.017.32344650-54
CCGTCTT456650.017.18060750-54
AAGAGCA1667400.016.0217326
AGCGATC466950.015.58145435-39
Produced by FastQC (version 0.11.5)