FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70403422
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCTCTATGTGTAGATCTC1594430.22647052582188404Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCTCTATGTGTAGATCT880430.1250550008776562Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG630250.040.283069
GAGCGTC700550.036.1785168
AGAGCGT760500.033.4218757
ATCGGAA832000.030.8885841
TCGGAAG840450.030.4770012
AAGAGCG853000.029.9590176
CGGAAGA871450.029.4510123
CGCCGTA329250.023.77680855-59
TCGCCGT349100.022.43315355-59
GATCGGA636300.021.7032681
GCCGTAT364150.021.53387655-59
GTCGCCG367100.021.31344455-59
TGGTCGC390300.020.28534950-54
GTGGTCG403450.019.68885250-54
CGTATCA395900.019.63938160-64
GAAGAGC1327050.019.6120385
TCTCGGT464750.017.3459645-49
CGCTCTA453000.017.29695130-34
GTGTCGC464600.016.8555525-29
GTCGCTC464450.016.79562230-34
Produced by FastQC (version 0.11.5)