FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176251
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT108170.4970474453544192No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT78870.3624122401322274TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT45820.21054556666487456RNA PCR Primer, Index 1 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTT41830.19221128445202323No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGT39700.18242381048877174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.14391722278358515No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT23700.1089028793094179TruSeq Adapter, Index 1 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTTCT22950.10545658566038568RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)