FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542127
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT195351.2667568883756006TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT176321.1433558974066338No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT111340.7219898231468614RNA PCR Primer, Index 1 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTT105850.6863896423576009No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGT71450.4633211142791742No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG67350.43673445831633845TruSeq Adapter, Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT66160.42901784353688116TruSeq Adapter, Index 1 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTTCT57850.3751312310853775RNA PCR Primer, Index 5 (95% over 21bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTTCTGCT35850.23247112591894184TruSeq Adapter, Index 5 (95% over 24bp)
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG28090.18215101609659903TruSeq Adapter, Index 1 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC16400.10634662385134298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15950.10342857624566588No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCGCGTATGCCGTCTTCTG15810.10252073921278856TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)