FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024477
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT224430.7420456495453593No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT153400.5071951282816831RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT128100.42354430203965837TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT126620.41865089402233835No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT89070.2944971973666852RNA PCR Primer, Index 39 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCT72900.24103340842069554RNA PCR Primer, Index 11 (95% over 23bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT48470.16025911256723063RNA PCR Primer, Index 39 (96% over 28bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCT35350.11687971176504236TruSeq Adapter, Index 11 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.11449913489175152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)