FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137299
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT333301.0623788169377544No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT263750.8406913080328016RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT220400.7025151252717704TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT201290.6416028564698487No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT152550.48624629020058335RNA PCR Primer, Index 39 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCT117800.37548222212801524RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG94790.30213887806039524RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT80310.2559845268174949RNA PCR Primer, Index 39 (96% over 28bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCT56440.1798999712810287TruSeq Adapter, Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG33150.10566413975843553Illumina PCR Primer Index 11 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)