FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459046
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT49631.0811552654853762No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT46451.0118811622364643RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT37730.8219219860319008TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT28710.6254275170680063RNA PCR Primer, Index 39 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT26590.5792447815687316No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCT21500.4683626477520771RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG17530.38187894023692615RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT16880.36771913925837496RNA PCR Primer, Index 39 (96% over 28bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCT11520.25095524195832225TruSeq Adapter, Index 11 (96% over 26bp)
TCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTGCTTG10960.23875602880757046TruSeq Adapter, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG6600.1437764407052888Illumina PCR Primer Index 11 (96% over 25bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA6510.1418158528774894RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.14116232360155626No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCTG5610.12220997459949548TruSeq Adapter, Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)