FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4297939
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT219690.5111519730736058No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT147880.34407189120180626RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT121340.2823213637978575No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT115340.2683611842792557TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT86480.20121272079478095RNA PCR Primer, Index 39 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCT63100.1468145546039625RNA PCR Primer, Index 11 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47890.11142549952430689No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT44480.10349146416456817RNA PCR Primer, Index 39 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)